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Penicillin works by inhibiting the synthesis of bacterial cell walls, specifically targeting the enzymes involved in forming peptidoglycan, a critical component of the bacterial cell wall. This disruption weakens the cell wall, leading to osmotic instability and ultimately causing the bacteria to burst and die. Penicillin is particularly effective against gram-positive bacteria, which have a thick peptidoglycan layer. By interfering with bacterial growth and replication, penicillin effectively treats various bacterial infections.

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