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There are now believed to only be five. Before 1995 there was widely believed to be about 24. Those five subspecies are the following:

1) Mackenzie Valley/Rocky Mountain/Alaskan Wolf (Canis lupus occidentalis)

2) Arctic Wolf (Canis lupus arctos)

3) Mexican Gray (Canis lupus baileyi)

4) Great Plains/Buffalo Wolf (Canis lupus nubilus)

5) Common Red Wolf (Canis lupus rufus)

Another Answer:Actually there is still considered to be around 24 subspecies of gray wolves in North America as of 2005, not 1995, with an addition of two more subspecies added to the list. These, including the 24 aforementioned, would be the following:
  • Kenai Peninsula Wolf (Canis lupus alces)
  • Newfoundland Wolf (Canis lupus beothucus)
  • Bernard's Wolf (Canis lupus bernardi)
  • British Columbia Wolf (Canis lupus columbianus)
  • Vancouver Island Wolf (Canis lupus crassodon)
  • Florida Black Wolf (Canis lupus floridanus)
  • Cascade Mountain Wolf (Canis lupus fuscus)
  • Gregory's Wolf (Canis lupus gregoryi)
  • Manitoba Wolf (Canis lupus griseoalbus)
  • Hudson Bay Wolf (Canus lupus hudsonicus)
  • Northern Rocky Mountains Wolf (Canis lupus irremotus)
  • Labrador Wolf (Canis lupus labradorius)
  • Alexander Archipelago Wolf (Canis lupus ligoni)
  • Eastern Wolf (Canis lupus lycaon)
  • Mackenzie River Wolf (Canis lupus mackenzii)
  • Baffin Island Wolf (Canis lupus manningi)
  • Mogollon Mountain Wolf (Canis lupus mogollonensis)
  • Texas Wolf (Canis lupus monstrabilis)
  • Yukon Wolf (Canis lupus pambasileus)
  • Alaskan Tundra Wolf (Canis lupus tundrarum)
  • Southern Rocky Mountains Wolf (Canis lupus youngi)f
  • Arctic Wolf (Canis lupus arctos)
  • Mexican Wolf (Canis lupus baileyi)
  • Domestic Dog (Canis lupus familiaris)
  • Mackenzie Valley Wolf (Canis lupus occidentalis)
  • Red Wolf (Canis lupus rufus)
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