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Generally during their second season (i.e. when they're on heat) around 18-24 months of age. After that it's best to give her at least one if not two seasons without pregnancy before mating again. Most labradors should only be mated/have puppies 4 times for optimum results and for the general health care of your dog. When she comes on heat, either take her to the vet for testing for optimum mating time (which can be done through a blood test to check her hormone levels) or around the 9th and 13th day mark of her 'season' if you are sure you know when she started.

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