There is a word "ancestors", meaning your family lineage or progenitors.
what is the proper way to address the mayor
Yes, 'ti-ti' is a Spanish equivalent of 'aunt'. But it's a particularly affectionate way of saying 'tia'. So perhaps a more correct English equivalent is 'auntie' or 'aunty'.
The proper way to address the conductor of a symphony orchestra is 'Maestro.'
The proper way to address an envelope for a Ph.D. is "Dr. [Full Name]" followed by their address.
the proper way to address the Queen is by "your majesty" or Ma'm, pronounced like "ham'
"Your honor"
usted. or ustedes.
Gentlemen
The proper way to address a Doctor of Osteopathic Medicine (DO) (not Doctor of Osteopathy) is Doctor (Dr.) since they are medical physicians.
There technically is no real word for it, but obachan is a familiar way of refering to a woman older than you and is commonly used as "auntie", whereas obasan is often used for "aunt".
If you wish to say My Mom you would say "Ma mere." For my aunt you would say "ma tante."