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Darkness represents sin in The Bible.

Additionally:

"Darkness" is the "natural state" of the human mind: selfish and hostile to authority; whether it be the laws of man or of God. It's a "slumbering human spirit" that's blinded [or deceived by the god of this world] to the Truth of God.

"...WAKE UP, for the coming of our Salvation will soon be here. So don't live in DARKNESS. Get rid of your evil deeds. Shed them like dirty clothes. Clothe yourselves with the Armor of Right Living, as those who LIVE IN THE LIGHT." (Rom.13:12 NLT New Living Translation)

"...In every willful sin, there is contempt of the Goodness of God. And though the branches of man's disobedience are very various, all spring from the same root. But in True Repentance, there must be a hatred of former sinfulness, from a change wrought in the state of the mind, which disposes it to Choose the Good and to Refuse the Evil. It shows also a sense of inward wretchedness. Such is the change wrought in Repentance, it is conversion, and is needed BY EVERY HUMAN BEING. The ruin of sinners is their walking [in darkness] AFTER A [natural] HARD AND IMPENITENT [darkened] HEART. Their sinful doings are expressed by the strong words, 'treasuring up wrath.' In the description of the just man, notice the full demand of the law. It demands that the motives shall be pure, and rejects all actions from earthly ambition or ends. In the description of the unrighteous, contention is held forth as the principle of all evil. The human will is in a state of enmity [hostility] against God [darkness]. Even Gentiles, who had not the written law, had that within, which directed them what to do by the light of nature. Conscience is a witness, and first or last will bear witness. As they kept or broke these natural laws and dictates, their consciences either acquitted or condemned them. Nothing speaks more terror to sinners, and more comfort to saints, than that Christ shall be the Judge. Secret services shall be rewarded, secret sins shall be then punished, and brought to Light." (Matthew Henry's Concise Commentary: Romans 2:1-16)

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