The secondary antibodies can bind to multiple sites on the primary antibody and thus produce a brighter signal since more dyes are brought to a single location.
http://www.bio.davidson.edu/COURSES/genomics/method/IMF.html
Advantages of indirect democracy include representation of diverse perspectives and the ability of elected officials to make informed decisions on behalf of the population. Disadvantages can include potential for corruption, lack of direct citizen participation in decision-making, and the risk of elected officials prioritizing their own interests over those of the people they serve.
With a direct fired system the airflow goes directly over the flame. An indirect fired air heater has a heat exchanger that the air passes over. Indirect units provides heat without contamination from combustion by-products.
Direct competition is a company that offers a product that customers may choose over your product. Indirect competition is a company that offers a substitute good.
Money that is received straight to the recipient over a period of time.
Direct characterization is when the author explicitly describes the character's traits and qualities, while indirect characterization reveals the character through their actions, thoughts, and dialogue. Direct characterization tells the reader what the character is like, while indirect characterization allows the reader to infer the character's traits based on their behavior and interactions with others.
Indirect democracy!
The British ruled directly over their colonies, while the French did not.
Direct corrosion of metallic materials occurs when the metal is in direct contact with a corrosive substance, leading to localized deterioration of the material. In contrast, indirect corrosion happens when the metal is exposed to a corrosive environment through a medium such as water or air, causing uniform degradation over a larger surface area. Direct corrosion is typically more rapid and localized, while indirect corrosion can be slower and spread out over a larger area. Both types of corrosion can weaken the metal and compromise its structural integrity over time.
The Philippines was under direct rule by the United States during the American colonial period (1898-1946). The direct rule meant that the U.S. government had direct control over the administration and governance of the Philippines.
Direct colonialism refers to a situation where a foreign power maintains control over a territory through the use of a local administration and military presence. Indirect colonialism involves exerting influence and control over a territory through economic, social, and political means without establishing a significant physical presence.
Indirect sunlight produces less heat compared to direct sunlight because it is diffused and scattered before reaching the surface. Direct sunlight carries more heat energy as it is more concentrated, whereas indirect sunlight spreads the heat over a larger area, resulting in less intense warmth.
Calculating an indirect cost rate involves several steps. First, identify and accumulate total indirect costs, which are expenses not directly tied to a specific project, such as administrative salaries and utilities. Next, determine the appropriate allocation base—often total direct costs or direct labor costs—over which these indirect costs will be spread. Finally, divide the total indirect costs by the chosen allocation base to derive the indirect cost rate, expressed as a percentage.