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The advantages and disadvantages of a broth tube culture compared to a culture on a Petri plate?
Advantages of broth tube culture: It allows for the growth of bacteria in a liquid medium which can facilitate the study of motility and oxygen requirements of bacteria, and it can provide a larger quantity of bacteria for testing. Disadvantages of broth tube culture: It can be difficult to isolate and identify specific colonies within a liquid medium compared to a solid medium like a Petri plate, making it challenging for colony counting and purity assessments. Additionally, broth tube cultures may not provide as clear visualization of bacterial growth compared to colonies on a Petri plate.
What is the procedural difference between using broth and agar as a source of bacteria for preparing a smear?
If your colonies were grown in broth, you can simply use your loop to collect loopfuls of liquid medium and smear that onto a glass slide. If they were grown on an agar plate you would have to add a few drops of water to the surface of the glass slide.
How could you tell the bacteria were growing in the culture?
Bacteria growth can be detected by an increase in turbidity (cloudiness) of the culture, formation of colonies on agar plates, or by changes in pH or color of the medium due to metabolic byproducts. Additionally, observing the presence of a pellicle, sediment, or turbidity in a liquid culture can indicate bacterial growth.
How do you grow bacteria in broth and argar?
To grow bacteria in broth, you would add the bacteria to a sterile liquid broth, incubate it at the optimal temperature for growth, and periodically check for bacterial growth by observing turbidity or colony formation. To grow bacteria on agar, you would spread the bacteria on a sterile agar plate using a spreader, incubate it at the optimal temperature, and observe colony formation.
What would you need to do to determine whether bacteria growing on a petri plate are anaerobes?
some sense
What are the advantages of liquid culture broth?
ne advantage of bacteria is that you can use them to make foods like cheese and bread. also , some bacteria is helpful, like they live in our bodies and they help protect us from disease. other bacterias cause disease, these are the bad bacteria. the type that can make people sick and perhaps even cause people to get so sick that they can die or maybe get really sick that they feel like they want to vomit on the floor
How would you change the bacterias plate to best tell if they are ampicillin resistant?
The best test would be to take some of the bacteria growing on the LB plate and streak them on a LB/amp plate. If the bacteria are viable on the LB/amp plate, then they are resistant to ampicillin. If no bacterial colonies survive, then they were not ampicillin resistant.
What would you need to do to determine whether bacteria growing on a petri plate from the brewer jar are anaerobes?
i. An anaerobic indicator. i. An anaerobic indicator. -anaerobic indicator, containing methylene blue, will turn white when oxygen is removed. if the bacteria grow while the anaerobic indicator is white then you know the bacteria is CAPABLE of anaerobic growth (growth in number, not size).
What are the advantages and disadvantages of plate girder bridge?
write it properly here
What does LB stand for in LB agar?
LB stands for Lysogeny Broth, which is a nutrient-rich medium used for cultivating bacteria. LB agar is a solidified form of this medium, containing agar to solidify the liquid broth for bacterial growth.
Advantages of pour plate method over other methods of bacterial colony?
The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.
Why are there areas on the agar jelly where no bacteria are growing?
Areas with no bacterial growth on agar jelly can be due to factors like competition with other bacteria, lack of necessary nutrients, inhibitory substances in the agar, or improper incubation conditions. Bacteria may also not grow in certain pH levels, temperatures, or oxygen concentrations.
