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To prepare a skin biopsy for direct immunofluorescence, a small skin sample is typically obtained using a punch or excisional biopsy. The specimen is then immediately placed in a suitable fixative, such as Michel's medium or a similar solution, to preserve the tissue architecture and antigenicity. After fixation, the sample is sectioned into thin slices and mounted on glass slides, ready for incubation with specific fluorescent antibodies that target the relevant antigens. The slides are then examined under a fluorescence microscope to detect any binding of the antibodies, indicating the presence of specific immune components in the skin.

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1w ago

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