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What is a sterile culture?
A sterile culture is a culture that is free from any living microorganisms. It is typically achieved through the use of sterile laboratory techniques and equipment to prevent contamination. Sterile cultures are important in microbiology and biotechnology for studying specific organisms or processes without interference from unwanted microbes.
What happens if we transfer the culture from an open broth tube into a sterile broth tube?
Transferring culture from an open broth tube to a sterile broth tube introduces microorganisms from the original culture into the sterile environment. If the transfer is done aseptically, the sterile broth will facilitate the growth of the introduced microorganisms, leading to an increase in their population. However, any contamination from the open tube could also introduce unwanted microbes, potentially affecting the purity and results of the culture. Proper aseptic techniques are crucial to minimize contamination during this process.
Why sterile conditions are needed in tissue culture?
The conditions that allow you to culture tissue are also ideal for the growth of bacteria, which given a chance will devour your tissue culture.
What is the Function of sterile mineral oil in the maintenance of stock culture?
To prevent moisture loss. A.C.
What is the function of sterile mineral oil in the maintenance of stock cultures?
Sterile mineral oil is used to create a barrier on the surface of the culture media in order to prevent the entry of contaminants and maintain a stable environment for the stock cultures. It helps to reduce the risk of contamination and dehydration of the cultures during storage.
How would you define pure culture sterile culture and contamination in the culture and isolation of bacteria?
A pure culture contains only one bacteria and no other. A sterile culture has only one bacteria in it and is usually used for transport. Contamination is something that doesn't belong. It can even be a small bit of plastic or wire. It is the opposite of a pure culture. The whole idea when working with isolating bacteria is to figure out which bacteria (or microbe) is causing an infection or disease in a patient. Your job is to help to figure this out so that the proper treatment can be started as soon as possible.
Why would a urine culture be needed?
A sterile container must be used to collect a urine culture for many reasons. The sample collected cannot be tainted with any substance that is being tested for.
Why should a nutrient medium be sterile?
A sterile nutrient medium is important to prevent contamination by unwanted microorganisms that may interfere with the growth of the desired organism being cultured. Contaminants can alter the experimental results and compromise the purity of the culture.
Is a flower sterile or non sterile?
the flower is sterile
If a sterile field becomes wet with sterile solution is the field no longer considered sterile?
If a sterile field becomes "contaminated" with a sterile solution, the field remains sterile.
What does a culture and sensitivity tube consists of?
A culture and sensitivity tube typically contains a sterile tube with a culture medium to support the growth of microorganisms. The tube is used to collect samples for culture testing, where the microorganisms are identified and tested against various antibiotics to determine the most effective treatment.
How do you take the sterile gauze from sterile bin?
sterile gloves
