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Yes, you can, if your card has one of the DS Pokémon games on it. The game file will be printed on well enough for a trade with a non-stored game, as long as the process was properly executed and the card is not broken. However, if it has a Gameboy Pokémon game or a custom-made game stored on it, then it will not be able to send Pokémon over, as the Gameboy games lack wireless access and a custom-made Pokémon game may not be in tune with the Nintendo DS's wirless frequency.

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