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Omitting sterile mineral oil in the decarboxylase test can lead to decreased sensitivity by allowing oxygen to enter the medium, which can inhibit the anaerobic conditions necessary for certain decarboxylase reactions. Without the protective layer of mineral oil, aerobic bacteria may outcompete anaerobic bacteria for nutrients, potentially leading to false-negative results. This can compromise the accuracy of the test in detecting decarboxylase activity in the target microorganisms. Thus, the absence of mineral oil can negatively impact the overall reliability of the test results.

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AnswerBot

2d ago

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How does adding the sterile mineral oil affect the specificity or the sensitivity of the Decarboxylation Test?

When you add mineral oil for Decarboxylation test you do not need a thick layer of oil, you only need enough to cover the surface of the medium. This promotes fermentation by locking out oxygen, and it also prevents false alkalinization at the surface of the medium. Hopefully my answer can help you. =)


What is the function of sterile mineral oil in the maintenance of stock cultures?

Sterile mineral oil is used to create a barrier on the surface of the culture media in order to prevent the entry of contaminants and maintain a stable environment for the stock cultures. It helps to reduce the risk of contamination and dehydration of the cultures during storage.


What is the Function of sterile mineral oil in the maintenance of stock culture?

To prevent moisture loss. A.C.


What is the difference between sterile distilled water and sterile water?

Sterile distilled water has no microbial contamination and is distilled and and sterile water merely has no microbial contamination.Distillation is the process of separation by heating a liquid until it evaporates and then condensing the steam back into a purer liquid as all contamination remains in the part which does not evaporate.


What does a culture and sensitivity tube consists of?

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Is it wfi used for inplace of lal reagent water?

The LAL reagent water can be sterile WFI or other water that show reaction with the specific LAL reagent with which it can be used, at the limit of the sensitivity of such reagent.


Is a flower sterile or non sterile?

the flower is sterile


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How do you preserve a sample for culture and sensitivity?

To preserve a sample for culture and sensitivity testing, it should be collected aseptically in a sterile container. The sample should be kept at the appropriate temperature and delivered to the lab promptly to prevent overgrowth of contaminating organisms. It is important to follow specific guidelines provided by the laboratory for proper preservation and transportation of the sample.


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Are sterile water and sterile distilled water the same?

no. Distilled water has had all chemicals and minerals removed, usually by steaming and re-condensation. Sterile water has been treated, usually heat treated, to destroy pathogens (any living organisms). So, you can sterilize distilled water, but not all distilled water is sterile. manufacturing criteria do NOT require that regular distilled water be packaged to prevent contamination. Water sold as sterile water must be packaged to avoid contamination. On the other hand, water full of minerals can be sterilized; the minerals are still present. So if you want mineral free, organism free water, you need sterile distilled; if just mineral free, distilled.