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dissolve in 95% ethyl alcohol and heat it

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Q: How do you make Sudan IV solution?
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What is Sudan iv solution?

it's a solution that is an indicator to test for lipids.


Importance of Sudan IV?

Like lipids, the chemical Sudan IV is not soluble in water; it is, however, soluble in lipids. Therefore to test for the presence of lipids in a solution you will use a Sudan IV Test. In this test dark red Sudan IV is added to a solution along with ethanol to dissolve any possible lipids. If lipids are present the Sudan IV will stain them reddish-orange, giving a positive test.


How Sudan IV detect the presence of lipid?

The chemical Sudan IV is not soluble in water; it is, however, soluble in lipids. Therefore to test for the presence of lipids in a solution you will use a Sudan IV Test. In this test dark red Sudan IV is added to a solution along with ethanol to dissolve any possible lipids. If lipids are present the Sudan IV will stain them reddish-orange, giving a positive test.


Sudan III is used to detect the presence of what macromolecules?

Sudan III is used to detect the presence of lipids.


Which test uncoated paper test or Sudan test is the most sensitive to small quantities of lipids?

for me, i think the uncoated paper test is more sensitive than Sudan iv test. This is because Sudan iv test can only soluble in certain solvent thus make it harder to define either the solution that is tested is really have lipid in it although there is not. The uncoated paper test can show if there is lipid the the solution if greasy transparent spot comes out from the uncoated paper.


Why distilled water is tested with Sudan IV in experiment of test for lipid?

because it want to show that Sudan iv is soluble in water n not soluble when there is lipid presence . From there you can make lipid as your responding variable


What happens when you mix Sudan Iv with oil only?

The Sudan IV dissolves into the oil making the oil red.


Why doesn't egg yolk have a reaction in the Sudan IV test?

Egg yolk should have a reaction in the Sudan IV test, because the Sudan IV tests for lipids (fats), and the yolk does have some fat in it. The Sudan IV test should have not had a reaction for egg membrane or egg white. Hope that helped!


What substance is used as a control in the Sudan iv test?

Usually water is the best control substance for the Sudan IV test.


Does a positive Sudan IV test means that the food is a significant source of lipids?

Sudan IV is a chemical that is only soluble in lipids. This indicates that a positive Sudan IV test means that the food is a significant source of lipids.


Is milk in Sudan IV soluble?

No


How do you use the Sudan IV test?

Sudan IV is a diazo dye for staining lipids, lipoproteins, and triglycerides. It is used in Sudan staining, wherein it attaches to the fats chemically.