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Polymorphic code was the first technique that posed a serious threat to virus scanners. Just like regular encrypted viruses, a polymorphic virus infects files with an encrypted copy of itself, which is decoded by a decryption module. In the case of polymorphic viruses, however, this decryption module is also modified on each infection. A well-written polymorphic virus therefore has no parts which remain identical between infections, making it very difficult to detect directly using signatures. Antivirus software can detect it by decrypting the viruses using an emulator, or by statistical pattern analysis of the encrypted virus body. To enable polymorphic code, the virus has to have a polymorphic engine (also called mutating engine or mutation engine) somewhere in its encrypted body. See Polymorphic code for technical detail on how such engines operate.[21]

Some viruses employ polymorphic code in a way that constrains the mutation rate of the virus significantly. For example, a virus can be programmed to mutate only slightly over time, or it can be programmed to refrain from mutating when it infects a file on a computer that already contains copies of the virus. The advantage of using such slow polymorphic code is that it makes it more difficult for antivirus professionals to obtain representative samples of the virus, because bait files that are infected in one run will typically contain identical or similar samples of the virus. This will make it more likely that the detection by the virus scanner will be unreliable, and that some instances of the virus may be able to avoid detection.

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