Bunsen -- not "bursen" -- the same who invented the Bunsen Burner.
Two light sources are compared by holding a slip of paper between them. A small oil or grease spot on the paper becomes nearly invisible from both sides when the intensity is equal. If a sliding card is mounted on a meter stick the distances can be measured. The intensity ratio is the square of the distance ratio. This only works with light of the same color. You should try this as it is easy to do and gives surprisingly good results.
results for the grease spot test
In the grease spot test, the filter paper becomes translucent when a grease spot is applied because the grease dissolves and spreads through the paper fibers. This causes the paper to become more transparent as the grease fills the spaces between the fibers, allowing more light to pass through.
Photometer is an equipment commonly used on a clinical laboratory. It has different kinds which can do certain jobs like testing blood samples. The examples of photometer used in a clinical laboratory are the flame photometer and Hemoglobin Photometer.
what are the principles behind LF Photometer
The flame photometer
unit of photometer
the steps for the grease spot test
The grease spot test is used to demonstrate the presence of lipids, which are fats and oils. When a substance containing lipids is placed on a piece of paper, it leaves a translucent grease spot after drying. This reaction occurs because lipids do not evaporate like water, resulting in a permanent mark on the paper. Thus, the grease spot test serves as a simple indicator for lipid content in a sample.
A grease spot forms on a piece of brown paper when a substance containing oil or fat is transferred onto the paper. The oil or fat is not absorbed by the paper and thus forms a visible spot.
One limitation of the grease spot test is that it may not be sensitive enough to detect small amounts of unsaturation in a sample. Additionally, the results of the test can be influenced by factors such as temperature and humidity, which can lead to inconsistencies in the results. Finally, the test may not be suitable for complex mixtures or samples with multiple components.
Photometer
A positive result in the grease spot test is indicated by the presence of a translucent, greasy spot on the test paper after a substance is applied and allowed to dry. This suggests the presence of lipids or fats in the sample. The spot will remain visible when held up to light, confirming the lipid content. This test is commonly used in food analysis and biological studies to detect fats.