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Since you have spark and other cylinders not popping up safe to assume not mixture problem. Check spark efficiency-see if a plug is loose, inspect gap, inspect boot to ensure good contact w/terminal inside, swap wire w/another close to the same length to see if cylinder moves. If you have recently changed plugs to another type with more resistance, that could do it as well. If a problem only occurs when motor hot that would tend to indicate electrical, since resistance increases across the board. A coil problem would most likely affect more than one cylinder, so can rule that out. Boot could be shorting, switching wire will tell you and if so some dialectic grease might solve your problem. Electricity always takes the path of least resistance. This will guarantee that the path is thru the end of the plug. If the electric looks good, check compression on that cylinder, if there is a valve problem and compression low on that one could cause misfire as well. Possibily since it is a 2000, I assume your miles are up there.

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