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A thin band is generally more desirable at the surface of Fluid Thioglycollate medium, as it indicates a higher concentration of aerobic organisms that are more likely to grow in oxygen-rich conditions. A thick band may suggest overgrowth or contamination, which can complicate the interpretation of microbial growth. The thin band allows for clearer differentiation of microbial growth patterns and better assessment of the organisms present.

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1mo ago

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Why is there a colored band at the surface of fluid thioglycollate medium which is more desirable a thick or thin colored band?

The higher viscosity of the Fluid Thioglycollate Medium prevents rapid uptake of oxygen and helps maintain the stratification of organisms growing in different layers of the broth.Oxygen is driven out of the broth by autoclaving, but as the broths sit at room temperature, oxygen begins to diffuse back into the tube. Any increase in the oxygen content is indicated by the redox indicator sodium resazurin which changes its color to red.Obligate aerobes will only grow in this oxygen-rich top layer. Obligate anaerobes will only grow in the lower areas of the tube. Microaerophiles will grow in a thin layer below the richly-oxygenated layer. Facultative or aerotolerant anaerobes can grow throughout the medium but will primarily grow in the middle of the tube, between the oxygen-rich and oxygen-free zones.


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