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Q: Is a thick band or thin band more desirable at the surface of Fluid Thioglycollate?
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Why is there a colored band at the surface of fluid thioglycollate medium which is more desirable a thick or thin colored band?

The higher viscosity of the Fluid Thioglycollate Medium prevents rapid uptake of oxygen and helps maintain the stratification of organisms growing in different layers of the broth.Oxygen is driven out of the broth by autoclaving, but as the broths sit at room temperature, oxygen begins to diffuse back into the tube. Any increase in the oxygen content is indicated by the redox indicator sodium resazurin which changes its color to red.Obligate aerobes will only grow in this oxygen-rich top layer. Obligate anaerobes will only grow in the lower areas of the tube. Microaerophiles will grow in a thin layer below the richly-oxygenated layer. Facultative or aerotolerant anaerobes can grow throughout the medium but will primarily grow in the middle of the tube, between the oxygen-rich and oxygen-free zones.


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