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Lots of reasons. If i nock my guitar against a shelf while i am playing, it can mess up the tuning and i have to retune it. also, humidity makes wood expand, and dryness makes it shrink, forcing compensation. I happen to know the oragn at my church is thrown way out of tune when the temperature changes between winter/summer and the metal shrinks/expands. The difference in the expansion/contraction change sthe way the air vibrates, so we have to compensate for it. also instruments get banged around before performance, whoch also puts them out of tune.

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