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The center of the band is used to calculate the RF value for a cation because it provides a more accurate representation of the overall migration of the ion during chromatography. The leading edge may be influenced by factors such as diffusion or interaction with the stationary phase, which can distort the true position of the cation. By using the center, you account for the spread of the band and obtain a more reliable measurement of the cation's mobility and separation efficiency.

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5d ago

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