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hatsune miku's best friend is most likely either luka or rin but since everything is fan made, it's up to the fans to choose her best friend.

another answer~ I think Rin because she was the 2nd Vocaloid plus i see alot of pictures with them together (as friends) buts its up to the fans really. Miku have lots of friends, she always hang out with them a lot, but all the time, she hangout with Rin, Len and luka.

What else can I help you with?