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Yes all media can be sterilized at the same temperature in order to insure that all pathogens have been killed and that only the one from a cultured sample will grow.

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Can all media be sterilize at the same temperature?

Yes all media can be sterilized at the same temperature in order to insure that all pathogens have been killed and that only the one from a cultured sample will grow.


Can all media be sterilized st the same temperature Why?

No, not all media can be sterilized at the same temperature due to variations in their composition and thermal stability. For instance, some media contain heat-sensitive components, like certain vitamins or proteins, that can degrade at high temperatures. Additionally, different types of media may require specific sterilization methods, such as autoclaving, filtration, or chemical sterilization, to effectively eliminate microbial contamination without compromising their integrity. Therefore, it's essential to choose the appropriate sterilization temperature and method based on the specific media being used.


Can culture media be sterilized in the oven?

No. Media sterilization requires 121C (250F) at 15psi for 15 minutes. It is generally done in an autoclave, but can be done in a pressure cooker. Tyndallization is another alternative, which is just boiling for 15 minutes three days in a row, and incubating at a warm temperature in between. It is not 100% effective. Instruments can be sterilized in an oven. Dry heat sterilization generally requires 160C (320F) for 2 hours or 170C (340F) for 1 hour.


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== ==


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