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Yes, enterococci can grow on CLED (Cystine Lactose Electrolyte Deficient) medium. CLED is designed to support the growth of a wide variety of urinary tract pathogens, including enterococci, while inhibiting the growth of certain contaminants. It allows for the differentiation of lactose fermenters from non-fermenters, which can help in identifying enterococci in clinical samples.

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1w ago

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How do you get rid of enterococci?

Some enterococci can be treated with penicillin, ampicillin, and vancomycin. Enterococci that are resistant to those drugs can be treated with Synercid.


Lab run out froom blood agar and cled plate what to do?

Depends on specimens you are inoculating - given CLED was mentioned I would assume urines. Standard set of PIM for a urine is HBA, MAC and CLED (although some labs omit the CLED). Most commonly encountered organisms in a UTI are enterobacteriaceae, especially E. coli; and other gram negative rods such as Pseudomonas. Can encounter Staphylococci and some other gram positive organisms - especially in hospital settings. Personally, in this situation I would use a CNA, Choc and MAC for urines - CNA grows the staphylococci (and streptococci and enterococci); Choc provides a non-selective media and MAC grows the gram negative rods that are most frequently isolated. Also, if nothing WAS isolated possibly release results with a comment about the accuracy of the results given the inavailbility of blood agar. Cled is useful for swarming proteus, to isolate them - I would attempt to isoalte and ID proteus without a CLED if necessary, if not put the plates aside (fridge) until a new supply of CLED can be brought in - if this will occur rapidly. If it will take longer consider shipping these specimens to another laboratory.


Why is urine specimen is inoculated on cled?

Urine specimens are inoculated on CLED (cysteine lactose electrolyte deficient) agar because this medium supports the growth of most urinary pathogens while inhibiting the growth of normal urinary flora. It allows for the differentiation of different bacterial species based on colony characteristics and lactose fermentation. CLED agar is also able to identify the presence of UTI-causing organisms such as E. coli, Proteus, and Enterococcus.


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