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Whole blood is a mixture of plasma, red blood cells, white blood cells, and platelets. Plasma is the liquid component of blood that carries nutrients and waste products, while red blood cells carry oxygen to tissues. White blood cells are part of the immune system, and platelets help with blood clotting.
Plasmapheresis is a procedure that involves removing blood from a person, separating out the plasma (liquid portion of the blood containing antibodies and other proteins), and then returning the remaining components of the blood back to the person. This process is often used to treat certain autoimmune disorders or conditions involving high levels of toxins or certain substances in the blood.
The whole blood is separated by the packing machine or sedimentation process.
Hematocrit test measures the percentage by volume of packed red blood cells in a whole blood sample. It is used to evaluate the concentration of red blood cells in the blood and can help diagnose conditions such as anemia or dehydration.
Plasma. Plasma is the fluid portion of whole blood that contains various proteins, including fibrinogen, which plays a crucial role in blood clotting.
Plasma? Serum?
The two main types of blood specimens used for most hematological tests are whole blood and plasma. Whole blood includes all components of blood, while plasma is the liquid portion of blood after cells have been removed.
Many medical tests require the blood to be centrifuged prior to analysis. However, the hematocrit is completed after only the centrifuging process. The hematocrit measures the volume of the formed elements of the blood as a percentage of whole blood volume.
Certain colored tubes, such as those containing EDTA (lavender or purple top tubes) or citrate (light blue top tubes), should not be centrifuged if they are being used for specific tests that require whole blood. Additionally, blood culture bottles should also not be centrifuged, as the presence of air and the integrity of the sample are crucial for accurate microbial growth. Always refer to specific laboratory protocols for guidance on handling different tube types.
The hematocrit is calculated by centrifuging whole blood to measure the volume of the formed elements. Many other lab tests require the blood to be centrifuged first, but the hematocrit is the test that can be done solely through centrifuging and measuring.
Blood for a folate test is typically drawn in a lavender-top tube, which contains the anticoagulant EDTA. The lavender-top tube is used for collecting whole blood specimens for hematology testing.
The buffy coat is a thin layer of white blood cells and platelets that forms between the plasma and red blood cells when whole blood is centrifuged. It primarily contains leukocytes (white blood cells), including lymphocytes, monocytes, neutrophils, eosinophils, and basophils, as well as thrombocytes (platelets). This layer is crucial for immune response and clotting functions in the body.
A buffy coat is a layer of white blood cells and platelets that forms when whole blood is centrifuged. It appears as a thin, pale yellowish layer between the plasma (top layer) and red blood cells (bottom layer) in a blood sample. The buffy coat is significant in laboratory diagnostics, as it contains the cellular components involved in immune response and clotting. It can be used for various analyses, including studying white blood cell populations and performing blood tests.
A lavender top tube is used for collecting whole blood specimens for analysis in hematology. The color of the tube top is a standardization for identifying the type of additive present in the tube for proper sample processing.
There is an interesting relationship between plasma and whole blood. Whole blood contains plasma but plasma does not contain whole blood.
Whole blood is generally used when a person has lost a lot of blood. Such blood loss can be caused by injury or surgical procedures. Whole blood is given to help restore the blood volume, which is essential for maintaining blood pressure.
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