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What is the function of the test reagent in oxidase test procedure?

In the oxidase test procedure, the test reagent serves to detect the presence of cytochrome c oxidase, an enzyme involved in the electron transport chain of certain bacteria. When the reagent, typically tetramethyl-p-phenylenediamine, is added to a bacterial culture, it reacts with the enzyme if present, resulting in a color change (usually to purple). This color change indicates a positive result, confirming the presence of oxidase-positive bacteria, while no color change suggests the absence of the enzyme.


What is chemical indicator dye?

Chemical indicator dye is a substance that changes color in the presence of a specific chemical or changes in pH. It is commonly used in laboratories to visually indicate the presence or absence of certain substances in a solution.


What test can be used to detect substance of emulsion?

A drop test can be used to detect the presence of an emulsion. Add a drop of the emulsion to water. If it disperses or forms a milky appearance, it indicates the presence of an emulsion.


Which nutrient is correctly paired with the indicator used to detect its presence?

Vitamin C - iodine solution is the indicator commonly used to detect the presence of vitamin C due to the color change reaction that occurs.


How will you know if the bacterial cells got transformed during the lab?

You can check for successful transformation by examining the bacterial cells under a microscope for any changes in morphology, performing a genetic assay such as PCR to detect the presence of the inserted DNA, or by plating the cells on selective media that only allow transformed cells to grow.

Related Questions

What receptor cells in the eye detect the presence or absence of light?

Rods


What detects the presence or absence of a mark in a predetermined place?

A sensor or detector is typically used to detect the presence or absence of a mark in a predetermined place. These devices can use various technologies such as photoelectric sensors, proximity sensors, or vision systems to detect the mark accurately.


What is the purpose of staining?

to detect bacterial stracture


What is the purpose of albert staining?

to detect bacterial stracture


What are photoelectric sensors used for?

Photoelectric sensors are commonly used for detecting the presence, absence, or distance of an object without physical contact. They are used in various applications such as factory automation, packaging, assembly line, and material handling to detect objects, count objects, and monitor processes.


What is the pupose of m9 paper?

to detect the presence of liquid nerve and blister agents to detect the presence of liquid nerve and blister agents


Can ants detect the presence of sugar through their sense of smell?

Yes, ants can detect the presence of sugar through their sense of smell.


Can sniffer dogs detect the presence of cocaine?

Yes, sniffer dogs can detect the presence of cocaine through their keen sense of smell.


What is the function of the test reagent in oxidase test procedure?

In the oxidase test procedure, the test reagent serves to detect the presence of cytochrome c oxidase, an enzyme involved in the electron transport chain of certain bacteria. When the reagent, typically tetramethyl-p-phenylenediamine, is added to a bacterial culture, it reacts with the enzyme if present, resulting in a color change (usually to purple). This color change indicates a positive result, confirming the presence of oxidase-positive bacteria, while no color change suggests the absence of the enzyme.


What is chemical indicator dye?

Chemical indicator dye is a substance that changes color in the presence of a specific chemical or changes in pH. It is commonly used in laboratories to visually indicate the presence or absence of certain substances in a solution.


How could you Detect the presence of cl-?

To detect the presence of CI- ion, add silver nitrate solution. A white opalescence to cloudy precipitate of silver chloride will form and confirm the presence of CI- ion.


What is the adaptive advantage of a nervous system to a free-living flatworm?

1. The nervous system allows a free-living flatworm to respond to stimuli in its environment. The eyespots in the nervous system can detect the presence or absence of light and the sensory cell can detect chemicals and movement in water. These adaptions are necessary to the primary environments of flatworms.