Describe how to carry out simple controlled experiments to illustrate how enzyme activity can be affected by changes in temperature?

Answer 1

· Add 2cm^3 of amylase to a test tube

· Add 2cm^3 of starch

· Add a few drops of iodine

· Place test tube into water bath at a certain temperature

· Observe colour changes every 2 minutes

· Record time taken for iodine to change back to yellow

· Repeat the experiment at different temperatures

Answer 2

1. Obtain an enzyme solution and substrate.
2. Set up multiple test tubes with the same enzyme and substrate concentrations but different temperatures (e.g. using a water bath).
3. Measure enzyme activity by observing changes in substrate concentration over time (e.g. using a colorimetric assay).
4. Compare the enzyme activity at different temperatures to see how it is affected by temperature changes.

Answer 3

Do you have mr lowe?...