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M smegmatis acid fast?
Mycobacterium smegmatis is a fast-growing species of mycobacteria, but it is not acid-fast like the pathogenic mycobacteria such as Mycobacterium tuberculosis. Acid-fast staining is a characteristic feature of mycobacteria that have a waxy lipid layer in their cell wall, which makes them resistant to staining by conventional methods.
Is Mycobacteria gram negative or positive?
If you have done your gram stain properly the mycobacterium will appear to be gram positive, however an acid fast stain is necessary because of the extremely long hydrocarbon chains and the high lipid content in the cell wall. The high lipid content makes the cell wall waxy. Both of these make the cell fairly impermeable to stains.
Is mycobacteria spore forming?
No, mycobacteria are not spore-forming bacteria. They are aerobic, nonmotile, and their cell walls contain mycolic acids, which make them resistant to many antibiotics and chemicals.
What chemical is responsible for the acid fast property of mycobacteria?
the cell wall contains mycolic acid. a dye (carbol fuchsin) is applied to the culture, then washed with acid-alcohol. those cells with mycolic acid in their cell wall will retain the dye even after the alcohol rinse. but those cells without mycolic acid will release the dye.
How do you think the acid fast nature of mycobacterium contributes to it virulence?
Mycobacteria grow slowly, are protected from lysis once phagocytized, are capbable of intracellular growth, resistant to gram staining, many antimicrobial drugs, and from desiccation(drying out). mycobacteria are hard to treat and require a long treatment
What do Mycoplasma sp and Mycobacteria sp.have in common?
Both stain poorly with the Gram stain.However Mycoplasma has no cell wall while Mycobacterium has a thick cell wall of mycolic acids.
Why are the mycobacteria so difficult to emulsify in water?
Mycobacteria have a unique cell wall structure that contains high levels of hydrophobic lipids, such as mycolic acids. These lipids make mycobacteria inherently resistant to being emulsified in water because they repel water molecules and do not easily mix with aqueous solutions. This hydrophobic nature is a key factor contributing to the difficulty in emulsifying mycobacteria in water.
M smegmatis acid fast?
Mycobacterium smegmatis is a fast-growing species of mycobacteria, but it is not acid-fast like the pathogenic mycobacteria such as Mycobacterium tuberculosis. Acid-fast staining is a characteristic feature of mycobacteria that have a waxy lipid layer in their cell wall, which makes them resistant to staining by conventional methods.
What is the difference between mycoplasmas and mycobacterium?
Mycobacteria are quite distinct from mycoplasma in a number of important ways. For example:Mycoplasma refers to a genus of bacteria that lacks a cell wall.Mycobacteria are acid-fast Gram-positive bacteria that have a very thick, protective, waxy cell wall.There are many other differences too. Here's how these organisms are classified:MycoplasmaOrder: MycoplasmatalesFamily: MycoplasmataceaeGenus: MycoplasmaMycobacteriaOrder: ActinomycetalesFamily: MycobacteriaceaeGenus: Mycobacterium
What is gram positive filamentous forms with mycolic acid in their cell wall?
It is most likely an actinomycete, a type of bacteria known for being filamentous with mycolic acid in their cell wall. Mycolic acid is a characteristic feature of the cell wall of certain bacteria, such as those in the mycobacteria group, including Mycobacterium tuberculosis.
Is Mycobacteria gram negative or positive?
If you have done your gram stain properly the mycobacterium will appear to be gram positive, however an acid fast stain is necessary because of the extremely long hydrocarbon chains and the high lipid content in the cell wall. The high lipid content makes the cell wall waxy. Both of these make the cell fairly impermeable to stains.
Is mycobacteria spore forming?
No, mycobacteria are not spore-forming bacteria. They are aerobic, nonmotile, and their cell walls contain mycolic acids, which make them resistant to many antibiotics and chemicals.
Does a plant cell have a cellwall?
Yes they do have cellulosic Cell walls; animal Cells utilize [glyco-protein] Cell coats.
What chemical is responsible for the acid fast property of mycobacteria?
the cell wall contains mycolic acid. a dye (carbol fuchsin) is applied to the culture, then washed with acid-alcohol. those cells with mycolic acid in their cell wall will retain the dye even after the alcohol rinse. but those cells without mycolic acid will release the dye.
Why does gram stain not work on mycobacterium tuberculosis?
Acid fast bacteria have a waxy coat on their cell wall, and their cell walls contain peptidoglycan. However, neither the crystal violet nor the counterstain (safranin) will penetrate the waxy layer. Therefore they will not be visible. An example of acid-fast bacteria are Mycobacteria. To visualize these bacteria, another staining technique called 'acid-fast staining' would be required.
How do you think the acid fast nature of mycobacterium contributes to it virulence?
Mycobacteria grow slowly, are protected from lysis once phagocytized, are capbable of intracellular growth, resistant to gram staining, many antimicrobial drugs, and from desiccation(drying out). mycobacteria are hard to treat and require a long treatment
Why is mycobacterium tuberculosis considered acid fast?
Saprophytic mycobacteria are acid fast and do not cause serious disease.
