Does dna ligase help assemble the leading strand?

Answer 1

Not in general replication as the leading strand is replicated straight away. The lagging strand is replicated in pieces that need ligase to affix them, one to the other.

However, in a replication bubble, when a lagging strand runs into a leading strand, and later the primers are replaced, ligase is still needed. Therefore, technically, the leading strand does need the ligase once.

Answer 2

No, DNA ligase does not help assemble the leading strand. DNA ligase is primarily involved in the final stages of DNA replication, where it seals the nicks in the phosphodiester backbone between Okazaki fragments on the lagging strand. DNA polymerase is responsible for assembling both the leading and lagging strands during DNA replication.

Answer 3

no