yes
Enzymes are not alive, meaning they cannot be killed.
No. Wash the lining, use a spray that will kill the fungus, or wash the shoe.
Freezing can slow down the growth of bacteria but may not always kill them. Some bacteria can survive freezing temperatures and become active again when thawed. To effectively kill bacteria, it is recommended to either use high heat (e.g. cooking) or certain chemicals (e.g. sanitizers).
Freezing should have an effect on amylase. Amylase is an enzyme, which is therefore a protein, and has optimum conditions. Freezing it will severely slow it down, and I'm pretty sure will denature it, so yes it will completely reduce if not stop the effect of amylase. Freezing does not denature enzymes, heat does.
No, honey is not the saliva of bees. Honey is made from nectar collected by bees from flowers, which is then stored, mixed with enzymes, and dehydrated in the beehive to create the thick, sweet substance we know as honey.
freezing provides unsuitable temperature for activities of enzymes
Yes, freezing temperatures can kill grass seed.
Freezing temperature make ice of the water in bacteria and completely stop there metabolic activity. Enzymes do not work at freezing temperature as they have no scope for movement in solid ice. So there activity halted.
Honey is considered a health food. It is loaded with beneficial nutrients and enzymes.
Freezing yogurt can reduce the number of live probiotics, but it does not completely kill them. The freezing process can damage some of the probiotics, leading to a decrease in their effectiveness.
Freezing meat does not kill bacteria, but it can slow down their growth. It is important to cook meat thoroughly to kill any bacteria present.
A bee uses its honey stomach to add various enzymes to the nectar that it has collected from flowers and turn it into honey.
Freezing yogurt can reduce the number of live probiotics present, but it does not completely kill them. The freezing process can damage some of the probiotics, leading to a decrease in their effectiveness.
Enzymes are not alive, meaning they cannot be killed.
The freezing point of honey is 40-50 0C. But honey may crystallize and under this temperature depending on a specific composition, content of water, presence of centers for nucleation, etc.
Freezing typically slows down enzyme activity by reducing the kinetic energy of the molecules, leading to a decrease in reaction rates. Boiling, on the other hand, denatures enzymes by disrupting the bonds holding the enzyme's three-dimensional structure together, effectively rendering the enzyme inactive.
No. Wash the lining, use a spray that will kill the fungus, or wash the shoe.