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How Can you elimintate gram positive bacteria from a gram positive gram negative mixture with mild detergent treatment?
as gram negative have low content of phospholipids than positive detergents at low concentration effectively kill negative bacteria
Gram negative bacteria are much more resistant to penicillin because?
Gram-negative bacteria are more resistant to penicillin due to the presence of an outer membrane that acts as a barrier, preventing the antibiotic from reaching its target (peptidoglycan layer). Additionally, gram-negative bacteria possess enzymes called beta-lactamases that can break down penicillin and render it ineffective. These mechanisms make it harder for penicillin to kill gram-negative bacteria compared to gram-positive bacteria.
How does streptomycin kill bacterial cells?
Streptomycin is a protein synthesis inhibitor. It binds to the S12 Protein of the 30S subunit of the bacterial ribosome, interfering with the binding of formyl-methionyl-tRNA to the 30S subunit. This prevents initiation of protein synthesis and leads to death of microbial cells. Humans have structurally different ribosomes from bacteria, thereby allowing the selectivity of this antibiotic for bacteria. However at low concentrations Streptomycin only inhibits growth of the bacteria by inducing prokaryotic ribosomes to misread mRNA. It is an antibiotic that inhibits both Gram-positive and Gram-negative bacteria and is a therefore a useful broad spectrum antibiotic
What is the importance of gram positive and gram negative bacteria?
Typical gram-positive bacteria are those staphylococci that produce boils; typical gram-negative bacteria are the bacilli that cause whooping cough; typical gram-variable bacteria are the bacilli that cause tuberculosis.
Why do some antibiotics work better against gram positive then gram negative bacteria?
Part of describing the function of an antibiotic is stating whether it is effective against Gram-positive bacteria, Gram-negative bacteria or both. (There are other aspects such as whether there is effect on aerobic versus anaerobic bacteria (those that can versus cannot survive in the presence of oxygen), but I'll address that some other time).Some antibiotics have broad spectrum activity, being able to kill Gram-positive and Gram-negative bacteria. At first that might seem like a good thing, and indeed it is when you are treating an infection with an unknown bacterium. However, broad spectrum activity is not desirable when you know exactly what bacterium you are trying to eliminate. If possible, it's much better to target treatment more specifically against the offending bacterium for various reasons, such as to reduce the risk of resistance emerging in other bacterial groups.Some antibiotics are narrow spectrum. Some are most effective against Gram-positive or Gram-negative bacteria, with little activity against the other. In general, we want to use an antibiotic of the most narrow spectrum possible.
What types of bacteria do antibiotics kill?
Gram negative and gram positive bacteria.
Does Tetracycline kill gram positive or gram negative bacteria?
Tetracycline kills both gram positive and gram negative bacteria. The mode of action to which Tetracycline works to kill bacteria is that it inhibits protein synthesis which works against both gram positive and gram negative bacteria.
How Can you elimintate gram positive bacteria from a gram positive gram negative mixture with mild detergent treatment?
as gram negative have low content of phospholipids than positive detergents at low concentration effectively kill negative bacteria
Gram negative bacteria are much more resistant to penicillin because?
Gram-negative bacteria are more resistant to penicillin due to the presence of an outer membrane that acts as a barrier, preventing the antibiotic from reaching its target (peptidoglycan layer). Additionally, gram-negative bacteria possess enzymes called beta-lactamases that can break down penicillin and render it ineffective. These mechanisms make it harder for penicillin to kill gram-negative bacteria compared to gram-positive bacteria.
How does streptomycin kill bacterial cells?
Streptomycin is a protein synthesis inhibitor. It binds to the S12 Protein of the 30S subunit of the bacterial ribosome, interfering with the binding of formyl-methionyl-tRNA to the 30S subunit. This prevents initiation of protein synthesis and leads to death of microbial cells. Humans have structurally different ribosomes from bacteria, thereby allowing the selectivity of this antibiotic for bacteria. However at low concentrations Streptomycin only inhibits growth of the bacteria by inducing prokaryotic ribosomes to misread mRNA. It is an antibiotic that inhibits both Gram-positive and Gram-negative bacteria and is a therefore a useful broad spectrum antibiotic
What is the importance of gram positive and gram negative bacteria?
Typical gram-positive bacteria are those staphylococci that produce boils; typical gram-negative bacteria are the bacilli that cause whooping cough; typical gram-variable bacteria are the bacilli that cause tuberculosis.
Why do some antibiotics work better against gram positive then gram negative bacteria?
Part of describing the function of an antibiotic is stating whether it is effective against Gram-positive bacteria, Gram-negative bacteria or both. (There are other aspects such as whether there is effect on aerobic versus anaerobic bacteria (those that can versus cannot survive in the presence of oxygen), but I'll address that some other time).Some antibiotics have broad spectrum activity, being able to kill Gram-positive and Gram-negative bacteria. At first that might seem like a good thing, and indeed it is when you are treating an infection with an unknown bacterium. However, broad spectrum activity is not desirable when you know exactly what bacterium you are trying to eliminate. If possible, it's much better to target treatment more specifically against the offending bacterium for various reasons, such as to reduce the risk of resistance emerging in other bacterial groups.Some antibiotics are narrow spectrum. Some are most effective against Gram-positive or Gram-negative bacteria, with little activity against the other. In general, we want to use an antibiotic of the most narrow spectrum possible.
Does negative ion kills bacteria?
Negative ions supposedly kill some bacteria. There is some debate on how effective this is and how many negative ions you have to have circulating to kill bacteria.
Why does streptomycin kill the bacteria and not us?
Streptomycin binds to bacterial 30S ribosomal subunit and inhibits protein production by the bacteria. Human beings are are not affected because our ribosomes have a 40S subunit and a 60S subunit, no 30S subunit.
Does penicillin kill gram negative or gram positive or both?
Penicillin primarily targets and is more effective against gram-positive bacteria by disrupting their cell wall synthesis. It is less effective against gram-negative bacteria due to the presence of an outer membrane that restricts penicillin's access to the cell wall.
Why do physician perform a gram stain on a sample before prescribing an antibiotic?
In order to know whose the best antibiotic to be prescribed. There are some antibiotics that are specific of a gram type (they won't be useful on both types), while there are other antibiotics that have a greater effect on a specific Gram type than on the other one.
Why gram negative bacteria more resistant to antibiotics?
Gram-negative bacteria have an outer membrane made of lipopolysaccharide and proteins, which the Gram-positive bacteria are lacking. More multi-drug resistance is being recognized in this class of bacteria than previously, and they are becoming a formidable foe in the environment because of the lack of new antibiotics to treat infections.
