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Why Low power objective before High Power objective?
The low power objective is used first for two reasons: First, focusing the low power objective will insure that the other objectives will be at the proper height. Smashing a slide with an objective can damage the objective. Second, you can see a greater area with the low powered objective. This allows you to easily locate an object of interest, center it in frame and then zoom in on it.
What is the difference of what you see under LPO or scanner with what you have observed under the HPO?
LPO (Low Power Objective) provides a wider field of view and lower magnification, making it suitable for locating objects and observing their overall structure. On the other hand, the higher magnification and narrower field of view of the HPO (High Power Objective) allow for more detailed examination of specific features or structures of the specimen. The scanner objective provides the lowest magnification and widest field of view for easily locating and navigating around the specimen on the slide.
How does letter e appear under low power objective?
Under a low power objective, the letter "e" would appear larger and more defined compared to a higher magnification. It may appear clearer and easier to discern the details of the letter.
Why is it that the image under the low power objective become inverted?
The image becomes inverted under the low power objective due to the design of the microscope and the way light rays are refracted and magnified by the lenses. The inverted image is a result of the optics in the microscope system.
What is the difference when observing a specimen under high power and low power?
use the fine adjustment knob to get a better view of what you are looking at.For low power you can use the coarse adjustment and fine adjustmentfor high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
How would you estimate the size of object viewed under high power objective lens 40 times if you were given the size of the field diameter when using low power objective lens 4 times?
You can estimate the size of the object by comparing the field diameters observed under the low power objective lens (4x) and high power objective lens (40x). Calculate the ratio of the field diameters (40x/4x = 10), and use this ratio to estimate the size of the object viewed under the high power objective lens. Simply multiply the size of the object viewed under the low power objective lens by the ratio (field diameter at 4x) to get an estimation.
Why must initial focusing of an object be under the low power objective and not under the high power objective?
Low power objective is like our eye if we want to see we can just see normally but if we want to see something very small but want to see properly at that particular part then we always go for some lens of something powerful. That's the same thing when we want to know something deeply we go for high power microscope to read a more detailed study of a particular part of object under the microscope.
What happens to the image of a focused object under LPO when the objective lens is shifted to HPO?
When an objective lens in a light-path objective (LPO) microscope is shifted from the Low Power Objective (LPO) to the High Power Objective (HPO), the image becomes magnified and the field of view decreases. This results in higher magnification but a smaller area of the specimen being visible at once.
Why Low power objective before High Power objective?
The low power objective is used first for two reasons: First, focusing the low power objective will insure that the other objectives will be at the proper height. Smashing a slide with an objective can damage the objective. Second, you can see a greater area with the low powered objective. This allows you to easily locate an object of interest, center it in frame and then zoom in on it.
How much the letter e you are viewing under the low power objective?
10x
What is the difference of what you see under LPO or scanner with what you have observed under the HPO?
LPO (Low Power Objective) provides a wider field of view and lower magnification, making it suitable for locating objects and observing their overall structure. On the other hand, the higher magnification and narrower field of view of the HPO (High Power Objective) allow for more detailed examination of specific features or structures of the specimen. The scanner objective provides the lowest magnification and widest field of view for easily locating and navigating around the specimen on the slide.
How much the letter e you are now viewing under the Low power objective?
10x
How much the letter e you are now viewing under the high power objective?
40x
When looking at a specimen for the fir ts time under a microscope do you view with high power objective or lowest objective fir ts?
first view with low power then gradually increase the power. hope it helped you!
Why is coarse adjustment knob not commonly used when focusing under high power objective?
The coarse adjustment knob moves the objective a lot while the fine adjustment knob moves it just a little, so using the coarse knob under high power can cause the objective to hit the slide and potentially damage the slide or the objective. The fine adjustment knob provides more precise control for focusing under high power, allowing for better clarity and accuracy.
How does letter e appear under low power objective?
Under a low power objective, the letter "e" would appear larger and more defined compared to a higher magnification. It may appear clearer and easier to discern the details of the letter.
Why is it that the image under the low power objective become inverted?
The image becomes inverted under the low power objective due to the design of the microscope and the way light rays are refracted and magnified by the lenses. The inverted image is a result of the optics in the microscope system.
