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Three reasons as to why: # Phosphorylation and the redox reactions are not directly coupled to each other, so the ratio of number of NADH molecules to number of ATP molecules is not a whole number. # The ATP yield varies slightly depending on the type of shuttle used to transport electrons from the cytosol into the mitochondrion. The mitochondrial inner membrane is impermeable to HADH so NADH in the cytosol is separated from the machinery of oxidative phosphorylation. Depending on the type of shuttle in a particular cell type, the electrons are passed either to NAD+ or to FAD in the mitochondrial matrix. 3. The use of proton-motive force generated by the redox reactions of respiration to drive other kinds of work.

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