Fungi staining using lactophenol cotton blue?

Answer 1

Lactophenol Cotton Blue is recommended for mounting and staining yeast and molds. It is formulated with lactophenol, which serves as a mounting fluid, and cotton blue. Organisms suspended in the stain are killed due to the presence of phenol. The high concentration of the phenol deactivates lytic cellular enzymes thus the cells do not lyse. Cotton blue is an acid dye that stains the chitin present in the cell walls of fungi.

Answer 2

el azul de lactofenol tiene tres funciones importantes a la hora de observar hongos del tipo mohos optenidos por aislamiento o de medios inoculados, el fenol destruye la flora acompañante, el acido lactico conserva las estructuras fungicas al provocar un cambio de gradiente osmotico con relacion al interior del fungico generando una pelicula por asi llamarlo protectora, pero el que realmente permite apreciar la estructura fungica es el azul de algodon el cual posee la capasidad de aderirse a la quitina presente en las hifas y conidios de los hongos microscopicos y tambien los macro pero es fundamentalmente utilizado en microscopicos u hongos inperfectos como aspergilius o penicillium

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translated by me:

lactophenol blue has 3 important functions when observing filamentous fungi. Phenol destroys contaminants compounds that has the fungi, the acid lactic conserves the fungi structures by provoking osmotic gradient change with relation of the interior of the fungi forming a protective "layer" (not really a layer i just could find the correct word to translate the other one..sorry I'm doing my best). The cotton blue is the one that gives the color and permit to visualize the fungi under optic microscope. it adheres to the chitin of the fungi walls of hiphas and conidios.

just to clarify: phenol + lactic acid + cotton blue = lactophenol cotton blue staining.

Answer 3

For the main part I would consider using the stain for fungal spores, particularly ones that are translucent or nearly so. Likewise, I use prefer Congo Red (if needed) to enhance cells within the actual fungus itself. RM

Answer 4

According to some Medical Technologists, LPCB is best and most commonly used in mycology department. LPCB is placed on an uncontaminated slide BEFORE placing the fungal culture because of that cultured fungi are alive and they needed to be killed as soon as possible to avoid possible abnormal side effect/s such as infection. As you can see, lactic acid or lacto preserves organisms while phenol kills organisms that would lead to arousal and isolation of fungal elements. With it's blue physical characteristic, it is easier to see spores or hyphae thus easier to identify presence of fungal elements.

Answer 5

Lactophenol cotton blue is a stain used to observe fungi under a microscope by highlighting their structures. The stain works by penetrating the fungal cell walls and staining the chitin found in the cell walls blue. This helps in identifying and differentiating fungal structures for classification and research purposes.

Answer 6

Phenol will kill any live organisms; lactic acid which preserves fungal structures, and cotton blue which stains the chitin in the fungal cell walls.

Answer 7

Staining of fungus by using lactophenol cotton blue. Where lactophenol acts as a cleaning agent and lactic acid acts as a preservative.

Answer 8

It allows you to visualize the hyphae. (I think it works by staining the chitin in the cell walls.)