How Can I Detect Sterol Glucoside By Using UV-vis Spectrometer (Dual Beam)?

Answer 1

To detect sterol glucoside using a dual beam UV-Vis spectrometer, first prepare a sample solution of the sterol glucoside in a suitable solvent. Measure the absorbance spectrum of the sample in the UV-Vis range (typically 200-400 nm) to identify characteristic absorption peaks associated with sterol glucosides. Compare the obtained spectrum with standard reference spectra to confirm the presence of sterol glucoside. Ensure to use a blank solvent to calibrate the spectrometer for accurate results.