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How a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
reporter enzymes can differentiate recombinants from non- recombinants on the basis of their ability to produce specific color in the presence of a chromogenic substrate.[ ref. insertional inactivation of beta-galactosidase when rDNA is inserted and formation of colored and non colored colonies accordingly].
What is the difference between natural and artificial transformation?
Natural transformation involves the uptake of foreign DNA by a cell through natural processes such as conjugation, transduction, or transformation, without any external intervention. Artificial transformation, on the other hand, is a laboratory technique that involves the deliberate introduction of foreign DNA into a cell using methods like heat shock or electroporation.
What does transformation and competent and plasmid mean?
Transformation is the process in which foreign DNA is introduced into a cell. Competent refers to a cell's ability to uptake foreign DNA during transformation. A plasmid is a small, circular piece of DNA that can replicate independently from the host cell's chromosome and is commonly used in genetic engineering for cloning genes or expressing proteins.
What is the process by which genes from one organism are transferred into DNA of another organism?
You would need to use Gene splicing to insert a foreign gene into an organism.
Which orginism is the better suited for total genetic transformation?
Bacteria are better suited for total genetic transformation because they have simple genomes and easily take up foreign DNA through processes like conjugation, transformation, and transduction. This makes it easier to manipulate and introduce new genetic material into bacteria for genetic engineering purposes.
How a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
reporter enzymes can differentiate recombinants from non- recombinants on the basis of their ability to produce specific color in the presence of a chromogenic substrate.[ ref. insertional inactivation of beta-galactosidase when rDNA is inserted and formation of colored and non colored colonies accordingly].
What is the role of calcium chloride in transformation?
Calcium chloride is used in transformation to destabilize the cell membrane, making it more permeable to foreign DNA. This helps in improving the uptake of the DNA by the cells, leading to successful transformation.
What is the process of adding foreign DNA to a bacterial cell called?
The process of adding foreign DNA to a bacterial cell is called Bacterial Transformation. It is a technique used very frequently in molecular Biology labs.Ê
What is the difference between natural and artificial transformation?
Natural transformation involves the uptake of foreign DNA by a cell through natural processes such as conjugation, transduction, or transformation, without any external intervention. Artificial transformation, on the other hand, is a laboratory technique that involves the deliberate introduction of foreign DNA into a cell using methods like heat shock or electroporation.
What does transformation and competent and plasmid mean?
Transformation is the process in which foreign DNA is introduced into a cell. Competent refers to a cell's ability to uptake foreign DNA during transformation. A plasmid is a small, circular piece of DNA that can replicate independently from the host cell's chromosome and is commonly used in genetic engineering for cloning genes or expressing proteins.
Which beat describes transformation in bacteria?
The process of bacterial transformation involves the uptake of foreign DNA by a bacterial cell and its incorporation into the bacterial genome. This transformation typically occurs naturally in some bacteria and can also be induced in a laboratory setting.
What is the definition of competent cells?
cells which readily accept a foreign DNA through a process called transformation
What is the process by which genes from one organism are transferred into DNA of another organism?
You would need to use Gene splicing to insert a foreign gene into an organism.
Which amendment protects a newspaper reporter who writes a story that is critical of the President's foreign policy?
Amendment 1
Why is heat shock important in bacterial transformation?
Heat shock is important in bacterial transformation because it helps the bacterial cells take up foreign DNA more efficiently. The sudden increase in temperature makes the cell membranes more permeable, allowing the DNA to enter the cells more easily. This increases the chances of successful transformation, where the foreign DNA is incorporated into the bacterial genome.
Compare the transformation of a bacterium cell with the transformation of a plant cell?
In bacteria, if the plasmid containing the foreign DNA manages to get inside a bacterial cell, this sequence ensures that it will be replicated. In Plant Cells, if transformation is successful the recombinant DNA is integrated into one of the chromosomes of the cell.
Which amendment applies to a News reporter who writes a story that is critical of the presidents foreign-policy?
amendment 1 baby
