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What are the indole test principles?
The indole test is based on the ability of certain bacteria to produce the enzyme tryptophanase, which converts tryptophan into indole. This indole is then detected by adding a reagent such as Kovac's reagent, which reacts with indole to produce a red color. A positive test indicates the presence of tryptophanase enzyme in the bacteria.
What color is negative indole test?
A negative indole test typically shows no color change, indicating the absence of indole production. The medium remains yellow or unchanged, suggesting that the tested organism does not possess the enzyme tryptophanase, which is responsible for converting tryptophan to indole.
What is the chemical formula of indole-3-acetic acid?
The chemical formula of indole-3-acetic acid is C10H9NO2.
What test will differentiate between Klebsiella pneumonia and Citrobacter freundii?
Biochemical tests such as indole test and citrate utilization can help differentiate between Klebsiella pneumoniae and Citrobacter freundii. Klebsiella pneumoniae is indole negative and citrate positive, while Citrobacter freundii is indole positive and citrate negative. Additional tests like urease and motility can also aid in differentiation.
What is the component in the SIM deep tubes that makes the medium suitable to detect the production of indole by bacteria?
The component in SIM deep tubes that makes the medium suitable to detect the production of indole by bacteria is tryptophan. Bacteria that possess the enzyme tryptophanase can break down tryptophan to produce indole, which can be detected by the addition of Kovac's reagent that reacts with indole to form a red color.
Why does ground beef sometimes smell sweet?
Ground beef can sometimes smell sweet due to the presence of bacteria that produce a compound called indole, which has a sweet odor.
What is an indole alkaloid?
class of alkaloids containing a structural moiety of indole.
What is the substrate for the Indole test?
The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to split indole from the amino acid tryptophan. This division is performed by a chain of a number of different intracellular enzymes, a system generally referred to as "tryptophanase."
What does the S.I.M. test for?
Sulfur Indole Motility test
What test will differentiate between Proteus vulgaris and Citrobacter freundii?
Indole test,Proteus vulgaris is be indole positive and Citrobacter freundii will be indole negative
What are the indole test principles?
The indole test is based on the ability of certain bacteria to produce the enzyme tryptophanase, which converts tryptophan into indole. This indole is then detected by adding a reagent such as Kovac's reagent, which reacts with indole to produce a red color. A positive test indicates the presence of tryptophanase enzyme in the bacteria.
Describe the smell of fresh tar?
Warm, oily and strong smell
What color is negative indole test?
A negative indole test typically shows no color change, indicating the absence of indole production. The medium remains yellow or unchanged, suggesting that the tested organism does not possess the enzyme tryptophanase, which is responsible for converting tryptophan to indole.
Is staphylococcus epidermidis indole positive?
S.aureus is a positive gram. Indole test is a part the biochemistry test to determinate a bacteria negative gram. indole test used to determine S.aureus, the result is negative. That is improve which S.aureus haven't trytophanase enzym (please see indole test principle)bcb
Is shigella indole positive?
Shigella species are generally indole negative, except for Shigella flexneri, which can be indole positive depending on the specific strain. In laboratory identification, indole testing is used to differentiate between various enteric bacteria. Therefore, while most Shigella species do not produce indole, Shigella flexneri may show positive results under certain conditions.
Indole test in vibro cholera?
The indole positive test is used to test the absence or presence of the vibro cholera.
Which is more basic pyrrole or indole?
Indole is more basic than pyrrole due to the presence of the nitrogen atom in the benzene ring of the indole structure. The lone pair of electrons on the nitrogen atom in indole makes it more basic compared to pyrrole, which lacks this nitrogen atom in the benzene ring.
