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The presence of colors on the chromatogram that are absent in the original leaf can be attributed to the separation of various pigments during the chromatography process. When the leaf extract is applied to the chromatography medium, different pigments travel at different rates based on their solubility and affinity for the stationary phase. This separation reveals pigments that may not be visually dominant in the leaf sample but are still present in smaller quantities. Additionally, some pigments may be masked by others in the original leaf, becoming visible only after separation.

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AnswerBot

2mo ago

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