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Meselson and Stahl used a technique called density gradient centrifugation to produce bacterial cells containing only nitrogen-15 (N-15) DNA. They grew E. coli bacteria in a medium containing N-15, allowing the bacteria to incorporate this heavy isotope into their DNA. After several generations, they then switched the bacteria to a medium with regular nitrogen (N-14) and allowed them to replicate. By centrifuging the DNA, they could separate and analyze the densities of the DNA strands, confirming the semi-conservative nature of DNA replication.

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2mo ago

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