How do I do the simplest form of chronological and replicative lifespan test for yeast?

Answer 1

To perform a simple chronological lifespan (CLS) test for yeast, first culture your yeast strain in rich medium until it reaches early stationary phase. Then, transfer aliquots of the culture to fresh medium without nutrients to induce stationary phase. Monitor the viability of yeast cells over time by performing serial dilutions and plating to count colonies, typically at regular intervals. For a replicative lifespan (RLS) test, use a microscope to track the number of daughter cells produced by individual mother cells, counting divisions until the mother cell stops dividing.