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Contractile vacuoles help maintain water balance in certain single-celled organisms, such as protozoa, by actively expelling excess water that enters the cell. These organelles collect water that diffuses into the cell due to osmotic pressure and periodically contract to expel the water outside the cell. This process prevents the cell from swelling and bursting, thereby maintaining osmotic stability and overall cellular homeostasis. Through this mechanism, contractile vacuoles play a crucial role in regulating the internal environment of the cell.

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