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Chromatography is the most used separation-technique in forensic-chemistry and forensic-toxicology. HPLC and thin-layer-chromatography are often used to analyse samples of blood and urine to search for drugs and toxines as well as to search for toxines in case of a murder or an unexplained death. To identify and quantify a unkown toxine chromatography is mostly combined with mass-spectrometry. But in case of searching for heavy-metals methods of atomic-absorption-spectrometry are more often used instead of chromtography.Gas-chromatography in connection with FID and headspace-analysis is a common technique to quantify the amount of alcohol in a blood-sample.

At least the technique to analyse DNA-samples called "Gel-Elektrophoresis" is also a chromatography-technique.

To analyse paints of faked paintings or documents or car paints in case of an accident or crimes, the IR-spectrometry is the more common technique, it would also be possible to analyse these samples by thin-layer-chromatography or other chromatography-techniques, but IR-spectrometry is more reliable.

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