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To avoid microbial contamination in a plant tissue culture lab, maintain strict aseptic techniques, ensure proper sterilization of equipment and growth media, maintain a clean work environment, and regularly monitor cultures for signs of contamination. Implementing a rigorous cleaning schedule and using fungicides or antibiotics when necessary can also help prevent microbial contamination.

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1y ago

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Why media culture is specifically?

Culture media should be specific to avoid contamination of unwanted microorganisms and to obtain a pure culture of microorganisms.


What is the purpose of flaming the loop before and after use?

Inoculating loop is used to inoculate microbial colony or sample on culture medium and to avoid the undesired microbial cells or to avoid contamination flaming of inoculating loop is necessary it is also called as incerination.


Why should cotton plugs of culture tube be always dry?

cotton plug on culture tube should be kept dry always to avoid contamination of the media by microorganisms which are suspended everywhere.


How do you avoid cross-contamination?

use a linear workflow


Why you should avoid allowing the loop filled with inoculate from touching the lip of either the source bottle or the bottle to be inoculated?

Touching the lip of the bottles can lead to contamination from airborne microorganisms. These microorganisms can affect the purity of the culture and compromise the results of the experiment. Additionally, it can introduce unintended microbes into either the source bottle or the bottle being inoculated, leading to unreliable outcomes.


Why are you advised to work as closely as possible to the flame?

to avoid contamination


Why do we need different toothpicks to mix the blood and serum on slides 1 and 2?

Two toothpicks are used to avoid cross contamination.


What is the purpose of a weight paper when using a scale?

To avoid contamination of the instrument.


How do you avoid contamination of reagents during experiments?

The best way to avoid contamination of samples in experiments is to limit contact with anything not necessary in the experiment. Specialized equipment may also help with this regard, such as the use of glass to avoid unwanted chemical reactions.


What is the purpose of wiping the laboratory bench top with disinfectant before you begin to handle culture?

Wiping the laboratory bench top with disinfectant before handling culture helps to remove any potential contaminants that may interfere with the work or compromise the integrity of the culture. It reduces the risk of contamination from environmental sources and ensures a clean surface for working with the culture.


Why to if you spill a substance or break any lab equipment notify your teacher immediately?

The motivations are: to avoid contamination, to avoid accidents, to avoid damages.


Why we have to Hold the test tube caps in the hand?

to avoid bench contamination answer by cowboy