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The melting temperature (Tm) of an oligonucleotide can be estimated using formulas like the Wallace rule, nearest-neighbor method, or online tools. These methods take into account factors like oligonucleotide length, GC content, and ionic conditions to predict the Tm based on the sequences' propensity to form stable base pairs. Experimental validation is often necessary to verify the calculated Tm.

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1y ago

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How do you calculate the tmfvalue?

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