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Size is easy. There are a number of techniques, some more accurate than others:
Mass spectroscopy - very accurate (to within ~0.1%). Requires a solution of about 1mg/ml.
Gel filtration chromatography - not particularly accurate (to within ~10%) and also influenced by shape.
SDS-PAGE - very crude estimation for proteins (commonly greater than 10% error) but based solely on molecular weight as molecules are denatured and given uniform charge.
Agarose gel - rough estimation for DNA.
Sequencing - protein/DNA sequencing would allow calculation of an approximate size, but it is much easier to use mass spectroscopy.
Analytical centrifugation - accurate but takes a very long time so rarely used.
A combination of the above methods can provide information on shape too. A typical early experiment on a protein, for example, might include both SDS-PAGE and gel filtration. Since one depends on size and shape, while the other is only size, one can determine its oligomer state (whether or not it binds to itself in solution) if it is known to be globular, or it can indicate if a protein is not roughly spherical (most are).
Shape is more complex:
As discussed above some basic information on shape (linear or globular and oligomerization) can be gained from the simple methods listed above. Further information can be gained through X-Ray crystallography, cryoelectron microscopy or COESY/NOESY 3D NMR spectroscopy. One of these techniques is usually able to give a reasonable high resolution three dimensional structure of a protein, but it is a time consuming and costly process.
For the very small biomolecules, mass spectroscopy and NMR spectroscopy (but not NOESY/COESY) are usually sufficient for determining both size and shape, as shape can be predicted using known bond length and angle constraints to a reasonable degree of accuracy.
What else can I help you with?
Which method can be used to gain the appropriate data for protein size and shape determinations?
This question was posed to me in my college biotechnology class this week. These are the answers that I have found. The only thing that a biotechnologist would use is the X-ray crystallography. Because it gives all the data needed to determine the size and shape of proteins. I do hope that helped.
In an isotonic solution what happens to the shape of the cell?
In an isotonic solution, the concentration of solutes inside and outside the cell is the same, so there is no net movement of water. This results in the cell maintaining its normal shape and size.
Is a magnets strength dependent on size or shape?
A magnet's strength is primarily dependent on the material it is made of and how it is magnetized. Size and shape can influence the distribution of the magnetic field and how it interacts with other magnets or objects, but they do not directly determine the strength of the magnet.
What properties of a plant does DNA help to determine?
DNA helps to determine the growth, development, and reproduction of a plant. It also influences the plant's traits such as color, size, shape, and resistance to diseases.
Does the size shape or form of your lips matter when kissing?
The size, shape, or form of your lips do not necessarily determine how good of a kisser you are. What matters more is technique, communication, and connection with your partner. Kissing is a personal and intimate act, and everyone has their own unique style and preferences.
Which method can be used to gain the appropriate data for protein size and shape determinations?
This question was posed to me in my college biotechnology class this week. These are the answers that I have found. The only thing that a biotechnologist would use is the X-ray crystallography. Because it gives all the data needed to determine the size and shape of proteins. I do hope that helped.
What 2 factors determine a pearls worth?
size, and shape
What are two factors that determine the proporties of a hydrocarbon?
This is a little vague, but size and shape?
What four factors determine the location size shape and direction of gyre?
The four factors that determine the location, size, shape, and direction of gyres are Earth's rotation, wind patterns, coastline shape, and water density. These factors contribute to the formation and circulation of gyres in the ocean.
What two factors determine the physical charactersistics of sediments?
The two factors that determine the physical characteristics of sediments are the size or grain size of the particles and the shape of the particles. Grain size refers to the diameter of the sediment particles, while shape refers to the angularity and roundness of the particles.
What describes a rock's texture geologists determine?
The size shape and pattern of the rocks grains
Does the shape or size of a ball effect the bounce of it?
Does the size of the ball determine how high the ball will bounce? I need the answer for a science project.
Does the size or shape of an animal determine whether an animal is classified as cold blooded or warm blooded?
The answer is no
What are the size shape and positions of the grain that makes up a rock?
These characteristics determine a rock's texture.
Shape and size of red blood cell when put in slty solution?
A red blood cell when placed in salty solution shrinks and becomes wrinkled.
Can a reflection be used to determine if figures are similar?
yes. if the two figures are the same size and shape, then they are similar
In an isotonic solution what happens to the shape of the cell?
In an isotonic solution, the concentration of solutes inside and outside the cell is the same, so there is no net movement of water. This results in the cell maintaining its normal shape and size.
