You would have to use a spectrophotometer to measure the absorbance of your unknown solution. But first, you need to make several solutions with known concentrations. Measure the absorbance of the known concentrations and plot them on an X and Y axis where X equals concentration and Y equals absorbance. Do a best-fit line for your data. Measure the absorbance of your unknown solution. Find this value on your Y-axis and find out where this value intersects with your line of best fit. The X value at the intersection is your concentration of potato cells. By the way, make sure you use the same wavelength throughout the experiment.
A 0.9% sodium chloride solution (normal saline) is more likely to be isotonic to the intracellular fluids of a potato. This concentration is similar to the overall solute concentration inside the cells of a potato, making it the best choice to prevent osmotic imbalance when the potato is immersed in the solution.
This is because the potato, which is mostly water, is in a hypertonic solution (a solution with less water and more solute --here, sucrose-- than the potato). Since the solutions want to reach equilibrium (equal amounts of sucrose and water in both the solution and the potato), water diffuses out of the potato and sucrose diffuses into it. The potato loses its water weight, and sucrose doesn't replace the weight lost, the potato weighs less.
A potato in salt water is hypotonic. This is because the salt water has a higher concentration of solutes compared to the potato cells, causing water to move out of the potato cells, leading to the cell shrinking.
Cells immersed in hypertonic solutions lose water and shrink, or crenate.
The movement of water into plant cells when they are immersed in distilled water is called osmosis. Osmosis is the diffusion of water molecules across a selectively permeable membrane from an area of higher water concentration to an area of lower water concentration.
A 0.9% sodium chloride solution (normal saline) is more likely to be isotonic to the intracellular fluids of a potato. This concentration is similar to the overall solute concentration inside the cells of a potato, making it the best choice to prevent osmotic imbalance when the potato is immersed in the solution.
In a hypertonic solution, potato strips lose water through osmosis, causing them to shrink and become shorter because the surrounding solution has a higher concentration of solutes than the potato cells. Conversely, in a hypotonic solution, water enters the potato cells, leading to swelling and an increase in length as the cells become turgid due to the lower concentration of solutes outside compared to inside the cells. This osmotic movement of water is driven by the concentration gradient of solutes.
When a potato is placed in water, the water concentration outside the potato cells is higher than inside. This creates a concentration gradient that drives water molecules into the potato cells through osmosis, causing the cells to swell and the potato to become turgid. The cell wall of the potato cells helps maintain the shape and prevents them from bursting.
During the potato osmosis experiment, the water molecules move from the area of higher concentration (outside the potato cells) to the area of lower concentration (inside the potato cells). This movement of water causes the potato to absorb water and become damp.
Isotonic solutions have the same concentration of solutes as the cells, while hypertonic solutions have a higher concentration of solutes than the cells.
This is because the potato, which is mostly water, is in a hypertonic solution (a solution with less water and more solute --here, sucrose-- than the potato). Since the solutions want to reach equilibrium (equal amounts of sucrose and water in both the solution and the potato), water diffuses out of the potato and sucrose diffuses into it. The potato loses its water weight, and sucrose doesn't replace the weight lost, the potato weighs less.
A potato in salt water is hypotonic. This is because the salt water has a higher concentration of solutes compared to the potato cells, causing water to move out of the potato cells, leading to the cell shrinking.
Hypertonic solutions are solutions that have a higher concentration than that of its immediate environment. The effects of hypertonic solutions on living cells is crenation in animal cells and plasmolysis in plant cells.
When a potato is placed in a concentrated sugar solution, water will move out of the potato cells via osmosis, resulting in the potato shrinking and becoming soft and limp. This is because the sugar solution has a higher solute concentration than the potato cells, causing water to move from an area of higher water concentration (inside the potato) to an area of lower water concentration (the sugar solution).
When a boiled potato is placed in a solution with a higher concentration of solute, water molecules will move out of the potato cells, causing it to shrink and become wrinkled. To improve this, you can immerse the boiled potato in a hypotonic solution, where the concentration of solute is lower than inside the potato cells, to allow water to move back into the cells, making the potato firm and hydrated again.
Cells immersed in hypertonic solutions lose water and shrink, or crenate.
You can place a piece of potato in a concentrated salt solution. Endosmosis will occur as water moves into the potato cells due to the higher concentration of solutes outside the cells. Conversely, placing the potato in distilled water will result in exosmosis, as water moves out of the cells to dilute the higher concentration of solutes inside the cells.