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How do you make FRAP reagent?
To make FRAP (Ferric Reducing Antioxidant Power) reagent, mix 25 ml of acetate buffer, 2.5 ml of TPTZ solution, and 2.5 ml of FeCl3 solution. Store the reagent in a dark container at 4°C until needed.
What is the equation for the preparation of ketones from secondary alcohols?
You can only make any ketone from any secondary alcohol by using PCC, Jones reagent, or NaCr2O4 in acid as a reagent.
How do you prepare nesslers reagent?
To prepare Nessler's reagent, dissolve 7g of mercuric iodide and 35g of potassium iodide in distilled water, and then add 100g of potassium hydroxide pellets. Once the solution is clear, dilute it with water to make 1 liter of Nessler's reagent. This reagent is commonly used for detecting ammonia in solutions.
What is a reagent strip?
The reagent strip is a strip of paper impregnated with a specific chemical reagent for a chemical determination.
What chemical reagent of protein?
Biuret reagent is used to test for protein in urine. It is a common test that students in biology class perform. Urine is added to a test tube, followed by approximately the same amount of Biuret reagent. If the solution turns lavender this means that there are proteins present in the urine.
Griess manual METHOD for nitric oxide?
The Griess assay is a simple colorimetric method used to quantify the levels of nitric oxide (NO) in a sample. In this method, nitrate and nitrite are first converted to nitrite by a nitrate reductase enzyme. The nitrite is then reacted with a Griess reagent to form a purple compound whose absorbance is measured at 540 nm. This absorbance is directly proportional to the concentration of NO in the sample.
What is the chemical formula for Greiss reagent?
1% Sulfanilamide 0.1% N-1-Naphthyl-ethylenediamine dihydrochloride 5% Phosphoric acid Made up with pure water
When did Peter Griess die?
Peter Griess died on 1888-08-30.
When was Peter Griess born?
Peter Griess was born on 1829-09-06.
What is the test for nitrogen oxide?
You need to be more specific, there is more than one oxide of nitrogen specifically dinitrogen monoxide, nitric oxide, dinitrogen trioxide, nitrogen dioxide, dinitrogen tetroxide, dinitrogen pentoxide and nitrogen trioxide. A start can be made by measuring its density. A mass spectrometer would do it.
What will you do if you transfer a reagent to another reagent?
i dont quite understand what your saying but if you mean using reagents to make a reagent like transmute bone. when you make the bone or whatever you will get rid of about 2 reagents to make one hard to find reagent to me not worth it. -Zarchary DayGlen on wizard101
I want to analyze nitrate and nitrite concentration in food products How can I prepare Griess reagent for spectrophotometric method?
why would you ask a question like this to the Internet? if you want to know you should probably contact a chemist or something...you need help. my answer to your question is...no one knows and that's why it hasn't been answered yet.
What is the best way to obtain 5ml of liquid reagent from a large reagent bottle?
Use a graduated pipette or syringe to measure and transfer 5ml of liquid reagent from the large reagent bottle. Make sure to carefully handle and dispense the reagent to avoid spills and contamination.
How do you make FRAP reagent?
To make FRAP (Ferric Reducing Antioxidant Power) reagent, mix 25 ml of acetate buffer, 2.5 ml of TPTZ solution, and 2.5 ml of FeCl3 solution. Store the reagent in a dark container at 4°C until needed.
Does the excess reagent determine the amount of a product?
No, the excess reagent does not determine the amount of product formed in a chemical reaction. The amount of product is determined by the limiting reagent, which is the reactant that is completely consumed first. The excess reagent will remain in excess after the reaction is complete.
How can one make a Wittig reagent?
To make a Wittig reagent, one can react an aldehyde or ketone with a phosphonium ylide. This reaction forms a carbon-carbon double bond, which is the key feature of a Wittig reagent. The resulting compound can be used in organic synthesis to convert carbonyl compounds into alkenes.
How do you test for presence of nitrite ion?
To test for the presence of nitrite ions, you can use the Griess test. This involves adding sulfanilic acid and N-1-naphthylethylenediamine dihydrochloride to the solution containing nitrite ions, which will produce a pink color if nitrite ions are present. Another method is using a diphenylamine reagent, which will turn blue in the presence of nitrite ions.
