How do you observe protein in a tissue?

Answer 1

PROTEINS ARE THE MACROMOLECULES COMPOSED OF AMINO ACIDS. EVERY PROTEIN HAS A PARTICULAR SEQUENCE OF THE AMINOACIDS CALLED THE PRIMARY STRUCTURE WHICH FOLDS TO PROVIDE THE PROTEIN A PARTICULAR 3D STRUCTURE. ON THIS 3D STRUCTURE LIES THE EPITOPES OR REGIONS THAT CAN BE IDENTIFIED BY THE ANTIBODIES OR IMMUNOGLOBLINS. TO VISUALIZE A PROTEIN IN A TISSUE ONE NEEDS TO HAVE PRIMARY ANTIBODY PARTICULAR TO THE PROTEIN IN QUESTION AND THE LABELED SECONDARY ANTIBODY SPECIFIC FOR THE PRIMARY ANTIBODY. ONE CAN USE FLUROSCENT MICROSCOPES TO VISUALIZE THE PROTEINS BY TAGGING THEM WITH A FLUROCHROME ON SECONDARY ANTIBODY. GAGANJOT

Answer 2

One way to observe protein in tissue is through techniques like immunohistochemistry or immunofluorescence. These methods utilize specific antibodies that bind to the protein of interest, allowing for visualization under a microscope. Another approach is Western blotting, where proteins are separated by size and then probed with specific antibodies to detect the protein within the tissue sample.