Recombinant organisms can be preserved by storing them in a cryoprotectant solution and freezing them at ultra-low temperatures using cryopreservation techniques. Another method is to preserve them in a lyophilized (freeze-dried) state, which involves removing water from the organism and storing it at a low temperature. Regular sub-culturing and maintenance of the organism's growth conditions are also important for long-term preservation.
a transgene is a gene that is not native to an organism (eg. it is a gene that is transferred from one organism to another)recombinant DNA is DNA that has essentially been combined with other, different DNA. DNA is double-stranded (the two strands are held together by basepair complementation). during recombination, the strands break apart, thus allowing another strand to bind with either of the original strands:original strandsDNA you want to insert into the organism (aka. a transgene) ]]you break apart the original strands | |and you add your strands |] |]now you have recombinant DNA, which is what a transgene will be when it has been integrated into an organism. it will (hopefully) be expressed correctly.(it's sliiightly more complicated than this haha, and the transgene can integrate (recombine) in numerous ways... but that's a solid foundational understanding of the process)hope this helps!NT
plasmids are vectors that are used to replicate recombinant DNA in a host organism or cell. they are normally used in cloning experiments.
In recombinant DNA technology, addition refers to the process of introducing a specific gene or DNA fragment into a plasmid or vector to create a genetically modified organism. This can involve inserting the gene of interest into the plasmid using restriction enzymes and ligases, allowing the gene to be expressed in the host organism.
Recombinant DNA is created by combining DNA from different sources, such as different species, through techniques like genetic engineering. Non-recombinant DNA refers to DNA that has not been modified in this way and only contains genetic material naturally found in an organism.
The host cell is important in recombinant DNA technology because it is the organism that will replicate the recombinant DNA construct. The DNA construct is inserted into the host cell, which then uses its machinery to produce the desired protein or molecule encoded by the inserted DNA. The choice of host cell is critical as it can affect the efficiency of DNA replication, protein production, and post-translational modifications.
Transgenic organism
recombinant DNA
Recombinant DNA is DNA this has been obtain from a source other then where it orginanated. In other words, a host has been used to create DNA from another organism.
a transgene is a gene that is not native to an organism (eg. it is a gene that is transferred from one organism to another)recombinant DNA is DNA that has essentially been combined with other, different DNA. DNA is double-stranded (the two strands are held together by basepair complementation). during recombination, the strands break apart, thus allowing another strand to bind with either of the original strands:original strandsDNA you want to insert into the organism (aka. a transgene) ]]you break apart the original strands | |and you add your strands |] |]now you have recombinant DNA, which is what a transgene will be when it has been integrated into an organism. it will (hopefully) be expressed correctly.(it's sliiightly more complicated than this haha, and the transgene can integrate (recombine) in numerous ways... but that's a solid foundational understanding of the process)hope this helps!NT
Recombinant vectors or Viral vectors are tools commonly used by molecular biologists to deliver genetic material into cells. This process can be performed inside a living organism (in vivo) or in cell culture (in vitro).
plasmids are vectors that are used to replicate recombinant DNA in a host organism or cell. they are normally used in cloning experiments.
In recombinant DNA technology, addition refers to the process of introducing a specific gene or DNA fragment into a plasmid or vector to create a genetically modified organism. This can involve inserting the gene of interest into the plasmid using restriction enzymes and ligases, allowing the gene to be expressed in the host organism.
Recombinant DNA is created by combining DNA from different sources, such as different species, through techniques like genetic engineering. Non-recombinant DNA refers to DNA that has not been modified in this way and only contains genetic material naturally found in an organism.
no
The host cell is important in recombinant DNA technology because it is the organism that will replicate the recombinant DNA construct. The DNA construct is inserted into the host cell, which then uses its machinery to produce the desired protein or molecule encoded by the inserted DNA. The choice of host cell is critical as it can affect the efficiency of DNA replication, protein production, and post-translational modifications.
A transgenic organism is an organism that has had a foreign gene inserted into its genome using biotechnology techniques. This process is used to introduce specific traits or characteristics into an organism that it does not naturally possess. Transgenic organisms are commonly used in research, agriculture, and medicine.
Transgenesis, which is the process of replicating DNA from one organism and inserting it into the DNA of another, creating what is called recombinant DNA