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To set up paper chromatography, begin by cutting a strip of chromatography paper and marking a baseline about 1-2 cm from one end using a pencil. Next, apply small spots of the sample solution along the baseline, ensuring they are spaced apart. Then, place the paper in a developing chamber with a shallow layer of solvent, ensuring the baseline is above the solvent level. Finally, cover the chamber to prevent evaporation and allow the solvent to rise up the paper, separating the components of the sample.

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4d ago

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Related Questions

What are the two types of paper chromatogaphy?

The two types of paper chromatography are ascending chromatography, where the solvent moves up the paper, and descending chromatography, where the solvent moves down the paper.


What is used as the stationary phase in paper chromatography?

The stationary phase in paper chromatography is the paper itself.


How is paper chromatography used in everyday life?

it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.


What are the key differences between paper chromatography and thin layer chromatography?

Paper chromatography and thin layer chromatography are both techniques used to separate and analyze mixtures of substances. The key differences between them lie in the materials used and the method of separation. In paper chromatography, a strip of paper is used as the stationary phase, while in thin layer chromatography, a thin layer of silica gel or other material is used. Additionally, in paper chromatography, the solvent moves up the paper through capillary action, while in thin layer chromatography, the solvent is applied directly to the stationary phase. Overall, thin layer chromatography is faster and more efficient than paper chromatography, but both techniques have their own advantages and applications in analytical chemistry.


What disadvantages are there in needing a reference set of chemicals?

because the chromatography paper is only short length


What can you use instead of chromatography paper in a paper chromatography test?

Instead of chromatography paper, you can use materials like coffee filters, filter paper, or even paper towels for paper chromatography tests. These alternative materials can absorb the solvent and help separate the components of a mixture based on their solubility and molecular properties, similar to chromatography paper.


Where may one learn about paper chromatography?

If you like science Paper Chromatography is something to look into. You can take Chemistry classes to find out different ideas on how to use Paper Chromatography.


Why does carotene travel farthest in chromatography of leaf pigments?

Carotene travels the farthest in chromatography of leaf pigments because it is the least soluble in the chromatography solvent. This means it interacts less with the solvent and more with the chromatography paper, allowing it to move further up the paper before the solvent front stops it.


What is paper chromatograph?

Ascending paper chromatography is where a chromatogram rises, or ascends. The paper moves up because the solvent moves it in an upward direction.


What is pen chromatography?

Pen chromatography is a simple paper chromatography technique where a capillary pen is used to apply a small sample to filter paper. As the solvent moves up the paper, different components in the sample will separate into distinct bands based on their interactions with the paper and the solvent. This technique is often used for separating and identifying components of a mixture.


Are paper and thin layer chromatography types of planer chromatography?

yes.


When using paper chromatography to separate screen methyl orange and methyl orange which one moves the furthest up the paper?

The Screened Methyl Orange moves the furthest