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Preparation of Glycol Chitin

Glycol chitin was obtained by acetylation of glycol

chitosan using a modification of the method of Molano et

al. (1979). Five grams of glycol chitosan was dissolved

in 100 mL of 10% acetic acid by grinding in a mortar, andthe viscous solution was allowed to stand overnight at

22°C. Methanol was added, and the solution was vacuum

filtrated through a Whatman No. 4 filter paper. The filtrate

wa s t r a n s f e r r e d i n t o a b e a k e r a n d 7 . 5 mL o f a c e t i c

anhydride was added. The gel was covered with methanol

and homogenized. The suspension was centrifuged at

27,000 g for 15 min at 4°C. The gelatinous pellet was

resuspended in 1 volume of methanol, homogenized, and

c e n t r i f u g e d a s i n t h e p r e c e d i n g s t e p . Th e p e l l e t wa s

resuspended in 500 mL distilled water containing 0.02%

(m/v) sodium azide (Trudel and Asselin, 1989).

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