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To determine if a protein is present or absent, techniques such as Western blotting, enzyme-linked immunosorbent assay (ELISA), or mass spectrometry can be employed. Western blotting involves separating proteins by size, transferring them to a membrane, and using specific antibodies to detect the target protein. ELISA quantifies proteins based on their ability to bind to specific antibodies in a sample. Mass spectrometry identifies proteins by measuring the mass-to-charge ratio of their ions, providing both presence and quantification.

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1w ago

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