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When a drug is administered, it is given at a prescribed dose so the active ingredient can bind and inhibit a particular enzyme, or target. Gene amplificatin increases the amount of copies of a particular area of chromosome and thus increases the amount of mRNA which will be transcribed. If the target is amplified over and over, it reaches a point to where the drug is not binding to enough of the substrate to be effective. Essentially, gene amplification dilutes a normal dose and in order to combat it, higher dosages are needed.
What else can I help you with?
Can plasmid DNA be used for 16srRNA amplification?
The 16s rRNA genes (rDNA) exist on genomic DNA. Therefore, plasmid has nothing to do with its amplification. However, if the 16s rRNA gene is cloned into the plasmid, it can be amplified.
What are three genetic changes that can convert proto-oncogenes to oncogenes?
Point mutations: Single nucleotide changes can lead to constitutive activation of proto-oncogenes. Gene amplification: Duplication of proto-oncogene sequences can increase gene expression levels, leading to oncogenic transformation. Chromosomal translocations: Rearrangements can cause fusion of a proto-oncogene with a new promoter region, resulting in abnormal activation.
What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
True or false cells containing a plasmid with an antibiotic resistance gene will die in the presence of the antibiotic?
False. Cells containing a plasmid with an antibiotic resistance gene will survive in the presence of the antibiotic because they can produce the protein that confers resistance, allowing them to withstand the antibiotic's effects.
What is the meaning of amplification of gene?
Gene amplification is the process of taking a very tiny sample (in some cases as few as one molecule of DNA) and rapidly generating a sample of millions or billions of identical molecules of DNA. This process must be entirely acellular, so that the sample is not contaminated with unrelated DNA. The most commonly used technique of gene amplification makes use of PCR (polymerase chain reaction) that makes use of a DNA polymerase enzyme derived from a virus. PCR only requires adding this enzyme and nucleotides to the DNA then cycling the temperature of the mixture up and down a little, each of these temperature cycles doubles the number of copies of the desired DNA molecule.
What words contain the root word gene?
gene alogical gene amdahl gene amplification
What is an amplificon?
An amplificon is a term used to describe a compound that enhances or amplifies a specific biological activity, such as gene transcription or enzyme activity. It is often used in the context of drug discovery and development to identify molecules that can increase the desired effect of a drug.
What is the specific primer sequence used in the PCR amplification of the target gene?
The specific primer sequence used in the PCR amplification of the target gene is 5'-AGCTGATCGATCGATCGATCG-3'.
What is a genotypic drug resistance test?
Genotypic testing can help determine whether specific gene mutations, common in people with HIV, are causing drug resistance and drug failure. The test looks for specific genetic mutations within the virus that.
What is the relationship between divergence and amplification?
Gene duplication (or chromosomal duplication or gene amplification) is any duplication of a region of DNA that contains a gene; it may occur as an error in homologous recombination, a retrotransposition event, or duplication of an entire chromosome.
Can plasmid DNA be used for 16srRNA amplification?
The 16s rRNA genes (rDNA) exist on genomic DNA. Therefore, plasmid has nothing to do with its amplification. However, if the 16s rRNA gene is cloned into the plasmid, it can be amplified.
What is a usually massive replication of genetic material and especially of a gene or DNA sequence?
amplification
Is p-glycoprotein a gene?
P-glycoprotein, also known as multidrug resistance protein 1 (MDR1), is a protein encoded by the ABCB1 gene in humans. It is a member of the ATP-binding cassette transporter family and plays a role in pumping out toxins and drugs from cells, contributing to drug resistance.
What can gene for antibiotic resistance be used for?
genetic marker
Where does new DNA come from in gene cloning?
New DNA molecules can come from various sources in gene cloning, such as PCR amplification of a specific gene, synthesis of a gene using recombinant DNA technology, or isolation of a gene from a donor organism. These DNA molecules are then inserted into a vector, such as a plasmid, to create a recombinant DNA molecule for cloning.
What is the cause of improper functions of a gene?
gene mutations
What are three genetic changes that can convert proto-oncogenes to oncogenes?
Point mutations: Single nucleotide changes can lead to constitutive activation of proto-oncogenes. Gene amplification: Duplication of proto-oncogene sequences can increase gene expression levels, leading to oncogenic transformation. Chromosomal translocations: Rearrangements can cause fusion of a proto-oncogene with a new promoter region, resulting in abnormal activation.
